AQA A-Level Biology: PCR (8.4.1) Lesson

This focused lesson explores PCR as an in vitro method for amplifying DNA fragments, part of the recombinant DNA technology process. Designed to meet point 8.4.1 of the AQA A-level Biology specification, it offers a clear breakdown of the method and its components.

Key Features:

• Introduction to PCR:

  • A quick quiz introduces the abbreviation and prompts students to recall the enzyme involved and its function.

• Step-by-Step Explanation:

  • Details the cyclical heating and cooling process, examining the temperatures and their significance in denaturation, annealing, and extension steps.

• Key Details Highlighted:

  • Explains the use of Taq polymerase, emphasizing its heat stability, and the role of primers in targeting specific DNA regions.

This clear and structured lesson ensures students understand PCR's mechanics and its importance in recombinant DNA technology.

• Exam Board: AQA
• Subject: Biology
• Level: A-Level
• Resource Type: Lesson
• Year: 2025
• Year Group/s: Year 12 and Year 13

This resource was created by GJHeducation