AQA A-Level Biology: Producing DNA Fragments (8.4.1) Lesson

This comprehensive lesson equips teachers to introduce the methods of producing DNA fragments as part of recombinant DNA technology. Designed to cover the first part of point 8.4.1 of the AQA A-Level Biology specification, it also lays the foundation for subsequent lessons on PCR and transformed host cells.

Key Features:

• Definition and Context: Starts with an introduction to recombinant DNA technology, linking to prior knowledge of the genetic code, transcription, and translation to explain how transferred genes function in transgenic organisms.
• Detailed Methods: Explores:
  • Use of reverse transcriptase and DNA polymerase, with discussions to reinforce enzyme roles and polynucleotide structure.
  • Restriction enzymes and their role in creating sticky ends, with a focus on restriction site structure.
  • Synthetic gene production using gene machines, highlighting applications of this advanced technology.
• Interactive Learning:
  • Quick quizzes to introduce and reinforce key terms like "cDNA" and "EcoR1."
  • Exam-style questions with mark schemes to build confidence and assess understanding.
• Application Focus: Encourages students to consider real-world uses of recombinant DNA technology through engaging application questions.

This lesson provides a solid framework for teaching the methods of DNA fragment production, integrating prior learning and preparing students for deeper exploration of recombinant DNA processes.

• Exam Board: AQA
• Subject: Biology
• Level: A-Level
• Resource Type: Lesson
• Year: 2025
• Year Group/s: Year 12 and Year 13

This resource was created by GJHeducation