AQA A-Level Biology: Transformed Host Cells (8.4.1) Lesson

This fully-resourced lesson guides teachers through delivering the third part of point 8.4.1 of the AQA A-Level Biology specification, focusing on the in vivo method for amplifying DNA fragments through the culture of transformed host cells.

Key Features:

• Step-by-Step Breakdown: Explains the five stages of transforming host cells, including:
  1. Preparing plasmids as vectors.
  2. Inserting DNA fragments into vectors.
  3. Transferring recombinant plasmids into host cells.
  4. Identifying successful transformations using marker genes.
  5. Replicating transformed cells to express the novel gene.
• Interactive Engagement: Incorporates a quiz to introduce key terminology like "vector" and reinforces recall of bacterial cell structures.
• In-Depth Exploration: Covers finer details such as the role of promoter and terminator regions, use of restriction enzymes, and the types of marker genes.
• Linked Knowledge: Continuously ties back to previous lessons on producing DNA fragments and the polymerase chain reaction, building student confidence in connecting sub-topics.
• Assessment Preparation: Ensures students are equipped to tackle exam-style questions requiring integration of knowledge across sections.

This lesson equips teachers with tools to deliver a clear, engaging exploration of DNA transformation, enabling students to master this fundamental biotechnology process.

• Exam Board: AQA
• Subject: Biology
• Level: A-Level
• Resource Type: Lesson
• Year: 2025
• Year Group/s: Year 12 and Year 13

This resource was created by GJHeducation